Journal: Scientific Reports

Article Title: DLGAP4 acts as an effective prognostic predictor for hepatocellular carcinoma and is closely related to tumour progression

doi: 10.1038/s41598-022-23837-y

Figure Lengend Snippet: Interfering with DLGAP4 inhibits the PPARβ/δ signalling pathway and the expression of proliferation- and metastasis-related proteins. Western blotting was performed to measure the protein expression of DLGAP4, PPARβ/δ, CyclinD1, E-cadherin and N-cadherin in shNC or shDLGAP4 HepG2 and HCCLM3 cells. The data were obtained from the average of three independent experiments. *P < 0.05.

Article Snippet: The following antibodies were used: rabbit PPARβ/δ monoclonal antibody (1:2000, Abcam, Cambridge, MA, USA), mouse β-actin polyclonal antibody (1:1000, Boster Biological Technology, Ltd), rabbit E-cadherin polyclonal antibody (1:1000, Boster Biological Technology, Ltd), rabbit N-cadherin polyclonal antibody (1:1000, Boster Biological Technology, Ltd), rabbit DLGAP4 polyclonal antibody (1:1000, Abcam, Cambridge, MA, USA), and rabbit CyclinD1 polyclonal antibody (1:1000, Boster Biological Technology, Ltd.).

Techniques: Expressing, Western Blot

Journal: Frontiers in Medicine

Article Title: Keratin 17 Suppresses Cell Proliferation and Epithelial-Mesenchymal Transition in Pancreatic Cancer

doi: 10.3389/fmed.2020.572494

Figure Lengend Snippet: K17 induced cell cycle arrest and cell apoptosis in pancreatic cancer cells in vitro . (A) Cell cycle distribution was analyzed by flow cytometry in stable K17 silenced SW1990 and CFPAC-1 cells and stable K17 upregulated HPDE6-C7 and PANC-1 cells. (B) Flow cytometry was used to determine the percentage of apoptotic cells in stable K17-silenced SW1990 and CFPAC-1 cells and in stable K17 upregulated HPDE6-C7 and PANC-1 cells. (C) Western blot analysis was performed to determine the expression of CyclinD1 and cleaved Caspase3 protein in stable K17-silenced SW1990 and CFPAC-1 cells and in stable K17 upregulated HPDE6-C7 and PANC-1 cells. * P < 0.05, and ** P < 0.01.

Article Snippet: The membranes were incubated at 4°C overnight with the following antibodies: rabbit monoclonal K17 (ab109725, Abcam, Cambridge, MA, UK), rabbit polyclonal cleaved Caspase3 (ab2302, Abcam, Cambridge, MA, UK), rabbit polyclonal CyclinD1 (26939-1-AP, ProteinTech Group, Inc., Wuhan, China) and rabbit polyclonal GAPDH (10494-1-AP, ProteinTech Group, Inc., Wuhan, China).

Techniques: In Vitro, Flow Cytometry, Western Blot, Expressing